Automated FISH (A-FISH)
The BV-SPERM-A-FISH includes the hardware and software needed to analyze automatically sperm cells by Fluorescence in situ Hybridization (FISH) technique. The system is optimized for use with all commercially available FISH probes.
The ability of the Duet system to scan and classify automatically thousands of cells per hour under fluorescent illumination, lends itself to the needs of the Andrology labs and IVF centers where 5000-10000 sperm cells from each ejaculate are analyzed.
Coupled with Solo – BioView’s offline analysis workstation – the Duet creates an effective and efficient tool for routine clinical use as well as research needs.
A screen with sperm cells scanned and classified automatically by the system as male sperm cells (Y, 18 genotype – one red and one aqua FISH signals). The sample was hybridized with an X,Y, 18 mix. The Pie graph in the left-hand corner shows the distribution of the targets according to their FISH pattern .
Target FISH (T-FISH)
Recent studies have shown that infertile male patients produce cytogenetically abnormal spermatozoa, despite a normal somatic karyotype, as a result of an altered intra-testicular environment that affects negatively the mechanisms controlling chromosome segregation during cell division. The rate of aneuploid spermatozoa production seems to be significantly higher in patients with teratozoospermia regardless of their sperm number and thus severely teratozoospermic men may be at an increased risk of producing aneuploid offspring. In extreme cases of absolute teratozoospermic patients, sperm with abnormal morphology were shown to have high incidence of aneuploidy. This association has been proven to be quite strong in individuals whose sperm cells had abnormal head morphology. In these cases chromosomal abnormalities were detected in virtually all spermatozoa and were largely attributed to an increase in sex chromosomes. The question of whether alteration of sperm morphology is always followed by chromosomal aberrations and reverse,still needs to be addressed in cases of mild and severe teratozoospermia, as morphology in addition to motility is one of the main selection criteria for identifying the sperm to be injected during the procedure of intra cytoplasmic sperm injection (ICSI). The association between the morphology of the individual spermatozoon and its chromosomal content was rarely checked until now due to technical difficulties while trying to preserve the stained cell shape after the performance of Fluorescence in situ hybridization (FISH).
The Duet Dual Mode Target– FISH (T-FISH) applications enables the scanning of slides of smeared ejaculate sperm cells stained with Eosin-Nigrosin staining (or other morphology staining) as the first step and then rescan the same slide after decondensation and applying FISH probe on it. The two scans are fully matched and for each and every cell in the sample the system can present two aspects of the same cell – morphology and FISH. The Dual Mode Application enables the user to analyze the genetic abnormality (FISH) of a pre-selected subset of sperm cells.
This is a unique and novel method to examine the potential relationship between chromosomal aberrations and morphology in the same spermatozoon.
Morphology and FISH images of the same sperm cell. The left image in each pair is of the sperm cell morphology and the right image shows the same cell with FISH. This screen shows all classes of sperm cells morphology with female genotype. (X, 18 – one aqua and one green FISH signal).
Pairs of two heads globozoospermic sperm cells showing diploidy in FISH (two aqua and two red FISH signals).
Pairs of amorphous heads (on the right side image) with disomy of the 18 chromosome in FISH (2 green FISH signals).