BioView Offers automated applications for the analysis of cells in blood and bone marrow samples. BioView Duet is able to scan either morphological and IHC stains – under bright light microscope, or FISH signals under Fluorescence illumination. The cells are identified according to their features. In the bright field mode the system automatically classifies the cells into 6 groups (PMN, lymphocytes, normoblasts, myelocytes, blasts and plasma cells) based on the cell morphology after MGG staining. After IHC staining the system finds automatically the positive stained cells. In the fluorescent mode it accomplishes fast and accurate spot counting and identifying other chromosomal aberrations such as, numerical aberrations, translocations, inversions etc. All of this is done at a very high rate (~10,000 cells per minute in bright field mode and thousands of cells per hour in fluorescent mode), in true color and high resolution.
Combined Analysis
Moreover, BioView has developed the software and the biological protocols enabling scanning a slide stained with Giemsa or labeled for the presence of a specific antigen by IHC, subsequently wash the initial stain, apply FISH probes of interest and rescan the slide on the Duet. The two scans are fully synchronized and for each and every cell in the sample the system can present two aspects of the same cell – morphology/IHC result, and the FISH signals of the probes that were applied to it. The combination of the two scans provides a full picture of a suspicious cell, and therefore increases the accuracy and specificity of the diagnosis.
The system also enables automated scanning and analysis of the FISH markers on pre-selected subset of cells based on morphology and/or IHC stain (Target FISH = T-FISH).
BioView has also developed the protocols to allow for performing FISH after FISH staining for cases where multiple FISH information is needed.
The power of the Duet system is in several aspects:
- Combination between two tests (genetic and morphology or genetic and IHC).
- Automating the present manual procedures thereby increasing the throughput and allowing lab personnel to do other tasks
- Enumeration of the cells of interest in an accurate manner.
- Automation helps to increase the Q.C. of the tests
- Increase the number of cells examined per case - hence reducing the false rates
- Increase sensitivity - in our systems we were able to detect:
- 1 x 10-5 cells in Brightfield scanning
- 5 x 10-6 cells in fluorescent scanning
- 1 x 10-4 cells using the Dual mode analysis (T-FISH)
- Increased specificity – by combining information of cellular markers and chromoshomal markers on the same cell the Duet significantly increases the specificity of the results.
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The Duet system has FDA clearance for the Hematology application
The Duet™ System is an automated scanning microscope and image analysis system. It is intended for in-vitro diagnostic use as an aiding tool to the pathologist in the detection, classification and counting of cells of interests based on color, intensity, size, pattern and shape.
The Duet™ System is intended to detect Hematopoietic cells stained by Giemsa stain, Immunohistochemistry or ISH (with brightfield and fluorescent) prepared from cell suspension. |
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